Neutrophils are specialized cells of the early innate immune response. A long-standing question in the field of neutrophil research is whether a distinct subset of these cells truly exists, or different populations are merely a manifestation of the neutrophil maturation/polarization state. In comparison to other myeloid cell types, lineage tracing experiments have been performed extensively to delineate distinct subsets of these cells; very little has been done for neutrophils. This talk will discuss how in-depth analysis of physiological and pathological granulopoiesis by multiomics and multiparametric technologies can contribute to better understanding neutrophil subsets discover new functions.