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SUMMARY:Uncovering the role of multivalency\, oligomerisation and dimensio
 nality in shaping biomolecular interactions\, function and regulation - Dr
  Roi Asor (Department of Chemistry and The Kavli Institute for Nanoscience
  Discovery)
DTSTART;VALUE=DATE-TIME:20251014T151500
DTEND;VALUE=DATE-TIME:20251014T161500
UID:https://talks.ox.ac.uk/talks/id/7cdef410-0ff7-4eda-8635-bf00ae2d1547/
DESCRIPTION:Biomolecular assembly is a cornerstone of cellular organisatio
 n and function. Understanding its principles is essential for both elucida
 ting biological processes and advancing therapeutic design. Multivalency\,
  the cooperative binding of oligomeric subunits to form higher order assem
 blies\, is a fundamental aspect of biomolecular interactions. Yet\, it rem
 ains challenging to quantify\, mainly due to the resultant molecular heter
 ogeneity. Here\, we present a mass photometry-based framework\, enabling r
 eal-time\, single-molecule visualisation of multivalent interactions in so
 lution and on lipid membranes. We apply it to SARS-CoV-2 spike-ACE2 intera
 ctions and inhibition\, as well as virus-like particle (VLP) assembly. We 
 show that SARS-CoV-2 infectivity and inhibition correlates with cooperativ
 ity and oligomerisation rather than 1:1 binding affinities. ACE2 promotes 
 spike oligomerisation in a variant-dependent manner\, while antibodies exp
 loit oligomerisation to enhance binding and inhibition. For VLPs\, weak mu
 ltivalent interactions drive hierarchical assembly through topologically s
 table intermediates consistent with the computed free energy landscape. Vi
 sualisation of individual assembly pathways events demonstrate that stocha
 stic\, state-dependent kinetics fine-tune the assembly process. Together\,
  our results establish a general experimental platform for resolving biomo
 lecular assembly and multivalent cooperativity controlling biological func
 tion with molecular resolution in real time.\nSpeakers:\nDr Roi Asor (Depa
 rtment of Chemistry and The Kavli Institute for Nanoscience Discovery)
LOCATION:Dorothy Crowfoot Hodgkin Building (Seminar Room 1 (Room 20-026))\
 , off South Parks Road OX1 3QU
TZID:Europe/London
URL:https://talks.ox.ac.uk/talks/id/7cdef410-0ff7-4eda-8635-bf00ae2d1547/
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DESCRIPTION:Talk:Uncovering the role of multivalency\, oligomerisation and
  dimensionality in shaping biomolecular interactions\, function and regula
 tion - Dr Roi Asor (Department of Chemistry and The Kavli Institute for Na
 noscience Discovery)
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