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SUMMARY:A novel nanowell system based on SmartChip™ technology for the i
 solation and processing of 1000s of single cells - Dr Maithreyan Srinivasa
 n (Wafergen)
DTSTART;VALUE=DATE-TIME:20150922T143000
DTEND;VALUE=DATE-TIME:20150922T153000
UID:https://talks.ox.ac.uk/talks/id/42bfc9e5-c6a8-47d5-909b-bb75455e17cf/
DESCRIPTION:: Advances in Next-Generation Sequencing have increased our ab
 ility to work with small/precious samples and grow our understanding of ba
 sic biological principles.  Such biological insights are typically derived
  from data “averaged” over bulk cell and tissue samples.  However\, si
 ngle cell analyses suggest that cell-specific transcriptome differences ma
 y have profound functional consequences.  While technologies to isolate an
 d process hundreds of single cells have made progress\, advancements in si
 ngle cell biology require new technologies that can enhance and automate t
 he isolation and processing of tens of thousands of individual cells in a 
 selective and flexible manner.  \n                To satisfy this need\, w
 e have engineered a single-cell system (SCS) to accommodate a number of do
 wnstream applications including RNA-seq.  The WaferGen SCS includes a 5184
  nanowell array based on SmartChip™ technology\, imaging station\, multi
 -sample nano-dispenser (for accurate dispense of cells and reagents into n
 anowells) and an image-analysis software that can identify 1000s of single
 -cell containing wells in an automated fashion.  Using Poisson dispense me
 thod\, the WaferGen SCIS can routinely isolate up to ~1800 viable single c
 ells. Analysis of acquired chip images by proprietary software quickly eva
 luates cell staining to assess cell viability.  The image analysis softwar
 e is then used to select viable isolated cells for further processing.  We
 ll locations of selected cell are then uploaded to the dispenser to add re
 agents into wells of interest. This unique selective processing of individ
 ual cell-containing wells can dramatically help to minimize data analysis 
 noise from wells containing multiple cells.\n                The new Wafer
 Gen SCS moves the science of single-cell analysis forward by offering:  Po
 wer – up to 1800 viable cells/chip across the broadest range of cell typ
 es per sample (5-100um)\, Control – ability to selectively process ONLY 
 single-cell containing wells and Insight - process up to 8 samples per chi
 p. We present data that demonstrate the reproducible dispensing of individ
 ual cells/nuclei using preprinted oligonucleotide-barcodes and processing 
 of  the cells for RNA-seq.\n\nSpeakers:\nDr Maithreyan Srinivasan (Waferge
 n)
LOCATION:Wellcome Trust Centre for Human Genetics (Room A)\, Headington OX
 3 7BN
TZID:Europe/London
URL:https://talks.ox.ac.uk/talks/id/42bfc9e5-c6a8-47d5-909b-bb75455e17cf/
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DESCRIPTION:Talk:A novel nanowell system based on SmartChip™ technology 
 for the isolation and processing of 1000s of single cells - Dr Maithreyan 
 Srinivasan (Wafergen)
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