Enhancing T cell affinity discrimination to reduce the cross-reactivity of T cell therapies
A single T cell receptor (TCR) can recognise up to one million different peptide ligands with varying affinities. We have developed a novel high-throughput Surface Plasmon Resonance (SPR) method to measure the affinity of hundreds of TCR/pMHC interactions in a single experiment. Using this method, we demonstrate that T activation correlates with ligand affinity. Furthermore, we have shown that modifying co-signalling receptors can selectively abolish T cell activation against low-affinity peptide. Overall, these findings demonstrate that T cells can be engineered to be less cross-reactive, which has important implications for enhancing the safety of adoptive T cell therapies.
Date:
2 June 2025, 13:00
Venue:
Online
Speaker:
Jose Cabezas Caballero (Dushek Group, University of Oxford)
Organiser:
Hashem Koohy (University of Oxford)
Organiser contact email address:
sally.pelling-deeves@medsci.ox.ac.uk
Host:
Hashem Koohy (University of Oxford)
Part of:
Unravelling T Cell Recognition: Insights from Immunology and AI
Booking required?:
Required
Booking url:
https://events.teams.microsoft.com/event/f3e9b4c6-dfe9-419c-8a5e-db867bfe6f92@cc95de1b-97f5-4f93-b4ba-fe68b852cf91
Cost:
Free
Audience:
Members of the University only
Editor:
Sally Pelling-Deeves
