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'Cryo Super-Resolution Fluorescence Microscopy for Correlation with Cryo-ET’
Super-resolution methods present a true game changer for the field of correlative light and electron microscopy (CLEM). They allow bridging the big resolution gap between conventional fluorescence microscopy (FM) and electron microscopy (EM). Cryo-immobilisation by fast-freezing techniques has been introduced to allow imaging in vitreous (glass-like) biological samples with superior structural preservation. On the one side, cryo-EM has evolved into a routine method for structural biology. Particularly cryo electron tomography (cryo-ET) offers insights into intact cells at unprecedented resolution. On the other side, super-resolution FM under cryo-conditions is still at a very early and experimental stage. However, the combination of both cryo-microscopy methods has great potential to open up a wide range of new application possibilities in structural and cellular biology. In my talk, I will discuss the challenges, our current solutions and the prospects for super-resolution cryo-CLEM.
Date:
10 January 2024, 14:30
Venue:
Dorothy Crowfoot Hodgkin Building, off South Parks Road OX1 3QU
Venue Details:
DCHB Seminar Room 2 (20-138)
Speaker:
Rainer Kaufmann (Centre for Structural Systems Biology, Hamburg, Germany)
Organising department:
Kavli Institute for Nanoscience Discovery
Organiser:
Lindsay Baker (University of Oxford)
Organiser contact email address:
lindsay.baker@bioch.ox.ac.uk
Host:
Lindsay Baker (University of Oxford)
Part of:
Seminar
Booking required?:
Not required
Audience:
Members of the University only
Editor:
Sarah-Jane Scard
