OxTalks will soon move to the new Halo platform and will become 'Oxford Events.' There will be a need for an OxTalks freeze. This was previously planned for Friday 14th November – a new date will be shared as soon as it is available (full details will be available on the Staff Gateway).
In the meantime, the OxTalks site will remain active and events will continue to be published.
If staff have any questions about the Oxford Events launch, please contact halo@digital.ox.ac.uk
Abstract:
Almost every eukaryotic mRNA has a 3’ polyA tail that contributes to post-transcriptional regulation of gene expression by regulating translation and mRNA stability. My laboratory studies the macromolecular protein complexes that control addition and removal of polyA tails: CPF, Ccr4–Not and Pan2–Pan3. We use a hybrid approach combining structural (cryo-EM, x-ray crystallography, NMR), biochemical, biophysical and genetic techniques to gain insights into their molecular mechanisms. I will discuss structural and mechanistic studies that have provided insights into the functions of these complexes. In addition, I will discuss recent advances in specimen preparation for electron cryo-microscopy (cryo-EM) that we are using to study these complexes.
