Visualising activation of the Fanconi Anemia DNA repair pathway
Status: This talk is in preparation - details may change
Status: This talk has been cancelled
Please contact the organiser if you would like to attend.
The Fanconi Anemia (FA) pathway repairs DNA damage caused by endogenous and chemotherapy-induced DNA crosslinks, and responds to replication stress. Genetic inactivation of this pathway impairs development, results in bone marrow failure and promotes cancer. The key molecular step in the FA pathway is the monoubiquitination of FANCD2-FANCI by the FA core complex-a megadalton multiprotein E3 ubiquitin ligase. Monoubiquitinated FANCD2 is thought to recruit enzymes to remove the DNA cross link or to stabilize the stalled replication fork. We recently reconstituted an active, recombinant FA core complex that efficiently monoubiquitinates FANCD2-FANCI in vitro and used electron cryo-microscopy (cryoEM) alongside mass spectrometry to determine the structures of several complexes. I will describe our efforts to understand how the proteins in the FA pathway signal and repair DNA crosslinks.
Date: 22 November 2021, 12:00 (Monday, 7th week, Michaelmas 2021)
Venue: Venue to be announced
Speakers: Speaker to be announced
Organising department: Department of Oncology
Organiser contact email address: annabelle.ziegler@oncology.ox.ac.uk
Booking required?: Not required
Audience: Members of the University only
Editor: Annabelle Ziegler