Physiological role and mode of regulation of mitochondrial Ca2+ signaling and metabolism interrogated by imaging and optometabolic control
The mitochondrial membrane potential powers both ATP production and mitochondrial Ca2+ transient of mitochondrial Ca2+ permeation into the mitochondria via the mitochondrial Ca2+ uniporter MCU followed by Ca2+ efflux by by the 3Na+/Ca2+ exchanger, NCLX that was discovered by our group . In the first part of my talk I will describe a new strategy for controlling the mitochondrial membrane potential. We are using optogenetic channels for a light dependent control in mitochondrial l membrane potential . In the first part of my talk I will described the challenges of targeting these “optometabolic channels to mitochondria and of using them to study metabolic processes, Ca2+ signaling and physiological activities. The second part on my talk will focus on our studies of the mitochondrial NCLX. . I will first describe how by combining molecular control of NCLX activity with cytosolic or mitochondrial Ca2+ imaging we study the role of NCLX in cell models related to diabetes Parkinson (PD) or pain sensation. I will then describe the mode of NCLX regulation by PKA phosphorylation. Finally I will present new data linking phosphorylation of NCLX to allosteric regulation of NCLX by mitochondrial membrane potential and describe the physiological implication of this regulation on thermogeneration in brown fat.
Date: 19 January 2018, 13:00 (Friday, 1st week, Hilary 2018)
Venue: Sherrington Building, off Parks Road OX1 3PT
Venue Details: Large Lecture Theatre
Speaker: Professor Israel Sekler (Ben-Gurion University of the Negev)
Organising department: Department of Physiology, Anatomy and Genetics (DPAG)
Organiser: Sally Collins (University of Oxford)
Organiser contact email address:
Host: Professor Anant Parekh (University of Oxford)
Part of: DPAG Head of Department Seminar Series
Booking required?: Not required
Audience: Members of the University only
Editor: Sally Collins