Mapping DNA replication stress in cancer cells and parasites with long-read sequencing and AI
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Every time a cell divides, it must replicate its genome exactly once, which it achieves through the parallel action of replication forks. The frequent slowing or stalling of replication forks, termed “replication stress”, is common in both cancer cells and parasites. Replication stress is therefore a common therapeutic target for anti-malarial and cancer chemotherapies, but we have a relatively poor understanding of where, when, why, and how often replication forks stall under these therapies. I will discuss our recent progress towards answering these questions, whereby we are using long-read nanopore DNA sequencing together with AI to measure the movement and stress of thousands of replication forks across the genomes of human cancer cells and malaria parasites.
Date:
1 November 2024, 13:00 (Friday, 3rd week, Michaelmas 2024)
Venue:
Medical Sciences Teaching Centre, off South Parks Road OX1 3PL
Venue Details:
Lecture Theatre
Speaker:
Dr Michael Boemo (University of Cambridge)
Organising department:
Sir William Dunn School of Pathology
Organiser:
Melissa Wright (Sir William Dunn School of Pathology)
Organiser contact email address:
melissa.wright@path.ox.ac.uk
Hosts:
Prof Peter Cook (Dunn School of Pathology),
Dr Anjali Hinch (University of Oxford)
Part of:
Dunn School of Pathology Departmental Seminars
Booking required?:
Not required
Audience:
Members of the University only
Editor:
Melissa Wright