Beauty and Benefits of cryo-EM; our new endeavors in vitrification of proteins and cells
We develop new methods for sample application and vitrification and implement them into a novel platform. The apparatus can be fed with pre-mounted autogrids, circumventing the need for postmounting. For single particle analysis (SPA), minute amount of samples can be applied directly onto a supported film. For cellular work, sample carriers with cells grown on them will be exposed to an airknife that removes excess medium. Sample evaporation will be minimized by a feedback loop that maintains the sample near its dewpoint calculated from the ambient temperature and humidity. Vitrification will be accomplished by jet-streaming liquid ethane onto the centre of the grid. I will be happy to share our latest results.
Date:
10 January 2017, 14:00 (Tuesday, 0th week, Hilary 2017)
Venue:
Wellcome Trust Centre for Human Genetics, Headington OX3 7BN
Venue Details:
Meeting Room A
Speaker:
Prof Peter Peters (Maastricht University)
Organising department:
Wellcome Trust Centre for Human Genetics
Organiser:
Eleanor Martin (Wellcome Trust Centre for Human Genetics)
Organiser contact email address:
stuart-pa@strubi.ox.ac.uk
Host:
Prof David Stuart (University of Oxford)
Part of:
Strubi seminars
Booking required?:
Not required
Audience:
Members of the University only
Editor:
Eleanor Martin
