Abstract: New global biodiversity targets focus on landscape and seascape connectivity as a foundational component of biodiversity conservation, including establishing “well-connected” networks of protected areas around the world. Recent advances allow the measurement and prediction of organismal movements at multiple scales and highlight how the resulting connectivity contributes to population persistence, ecological function, and ecosystem services. This knowledge can guide biodiversity conservation, ecological restoration, and climate adaptation, yet much of connectivity science remains to be fully integrated into planning. Given the rapidly closing window of opportunity to conserve connectivity, a global effort is required to align and mainstream the scientific knowledge produced with the pressing need to map, protect, and restore areas that support species’ movements and meet the diverse needs of nature and people. In this talk, I will provide key evidence on the importance of connectivity for species persistence and biodiversity, new unified and scalable models that honor species movement while making reliable predictions for connectivity, and a generalized framework for connectivity conservation to promote ‘well-connected’ landscapes.