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Solution-phase molecular interactions and time-resolved multiple biophysical parameters can determined in a single experiment,
making native mass spectrometry the most powerful benchtop analytical technique available in protein science. In the sixth seminar of CMD’s mass spectrometry training series, Rod Chalk describes practical aspects of the technique, and shows how electrospray ionisation charge states are determined by the physical properties of a protein, rather than its solution phase chemistry. He will go on to show how the technique has been automated making it the ideal tool for high throughput fragment screening.
