A Human Liver Cell Atlas: Revealing Adult Liver Progenitors by Single-Cell RNA-sequencing
If you would like to speak to Dominic before his talk contact edward.morrissey@imm.ox.ac.uk
The human liver is an essential multifunctional organ, and liver diseases are rising with limited treatment options. However, the cellular composition of the liver remains poorly understood. Here, we performed single-cell RNA-sequencing of ~10,000 cells from normal liver tissue of 9 human donors to construct a human liver cell atlas. Our analysis revealed previously unknown sub-types among endothelial cells, Kupffer cells, and hepatocytes with transcriptome-wide zonation of some of these populations. We reveal heterogeneity of the EPCAM+ population, which comprises hepatocyte-biased and cholangiocyte populations as well as a TROP2int bipotent progenitor population with strong potential to form liver organoids. As proof-of-principle, we utilized our atlas to unravel phenotypic changes in hepatocellular carcinoma cells and in human hepatocytes and liver endothelial cells engrafted into a mouse liver. Our human liver cell atlas provides a powerful resource enabling the discovery of previously unknown cell types in the normal and diseased liver.
Date: 7 March 2019, 16:00 (Thursday, 8th week, Hilary 2019)
Venue: MRC Weatherall Institute of Molecular Medicine, Headington OX3 9DS
Venue Details: Seminar Room
Speakers: Speaker to be announced
Organising department: MRC Weatherall Institute of Molecular Medicine
Organiser: Dr Ed Morrissey (University of Oxford)
Organiser contact email address: edward.morrissey@imm.ox.ac.uk
Host: Dr Ed Morrissey (University of Oxford)
Part of: WIMM Occasional Seminars
Booking required?: Not required
Audience: Members of the University only
Editor: Sarah Butler