Peptide exchange on MHC class I molecules mediated by small molecules
MHC class I-peptide complexes are usually stable for hours or even days. We have found that small molecules – for example certain dipeptides – that bind into the F pocket can dramatically accelerate peptide dissociation and replacement with exogenous peptides. This works on recombinant class I molecules, where the technology can be used to rapidly manufacture MHC multimers for T cell staining, and on cell surfaces, from which peptides can now be natively eluted.
Date: 23 June 2015, 14:30 (Tuesday, 9th week, Trinity 2015)
Venue: MRC Weatherall Institute of Molecular Medicine, Headington OX3 9DS
Venue Details: Seminar Room
Speaker: Prof Sebastian Springer (Jacobs University Bremen, Germany)
Organising department: MRC Human Immunology Unit
Organiser: Anne Farmer
Organiser contact email address:
Host: Alain Townsend
Booking required?: Not required
Audience: Members of the University only
Editor: Anne Farmer