Genomic regulatory architecture in human stem cell commitment and heamopoiesis
Three-dimensional chromatin organization plays a major role in metazoan gene regulation. However, the dynamic nature of genomic architecture across cell types and its effect on lineage commitment is still not fully understood. In collaboration with other groups at Babraham Institute and beyond, we are using Promoter-Capture Hi-C to identify distal sequences interacting with ~22,000 promoters in human cells globally and at high resolution.
My talk will focus on two projects:
(1) the analysis of interactome rewiring upon human ES cell commitment and
(2) the profiling of promoter interactions in 17 primary haemopoietic cell types and using this information for linking common disease variants with putative target genes and pathways.
Taken together, the results of these projects highlight the power of high-resolution interactome profiling for gaining insights into both global gene regulatory principles and the mechanisms underlying specific disease pathologies.
Date: 10 March 2016, 14:00 (Thursday, 8th week, Hilary 2016)
Venue: Old Road Campus Research Building, Headington OX3 7DQ
Venue Details: 71ab
Speaker: Dr Mikhail Spivakov (Babraham Institute)
Organising department: CRUK Oxford Centre
Organisers: Anastasia Samsonova (University of Oxford), Christopher Yau (University of Oxford)
Organiser contact email address: anastasia.samsonova@oncology.ox.ac.uk
Hosts: Anastasia Samsonova (University of Oxford), Christopher Yau (University of Oxford)
Part of: Cancer Bioinformatics Seminar Series
Topics: Functional genomics, Promoters, Epigenetics, Cancer, Hematology, Transcription, Bioinformatics, Bioinformatics--Methodology, Genomics
Booking required?: Not required
Audience: Members of the University only
Editor: Anastasia Samsonova