Understanding regulatory circuitry through large-scale genetic perturbation analyses
The long-term goal of the Frank Holstege’s group is to develop wiring-diagram models that describe how regulatory circuitry works at the molecular level across entire genomes. Their initial focus is on transcription regulation and their analyses require quantitative measurements of transcription rates for all genes, determination of transcription factor occupancy across the genome, mechanistical insight into signal transduction induced protein-modifications and their consequences for transcription, analysis of chromatin structure and modifications, the activity of co-regulatory protein complexes, etc. They have put together a powerful tool-box for genome-wide analyses that includes DNA microarray expression-profiling, genome-wide localisation analysis by ChIP on chip and various bioinformatics tools.
Date: 21 April 2016, 11:00 (Thursday, 0th week, Trinity 2016)
Venue: NDM Building, Headington OX3 7FZ
Venue Details: Basement Seminar Room
Speaker: Prof Frank Holstege (University Medical Center, Utrecht)
Organising department: Ludwig Institute for Cancer Research, Oxford Branch
Organisers: Mary Muers (Oxford Ludwig Institute, NDM Experimental Medicine), Alexandra Ward (University of Oxford, Oxford Ludwig Institute, NDM Experimental Medicine)
Organiser contact email address: alexandra.ward@ludwig.ox.ac.uk
Host: Dr Sebastian Nijman (Ludwig Cancer Research, Oxford)
Part of: Ludwig Institute Seminar Series
Booking required?: Not required
Audience: Members of the University only
Editor: Mary Muers