OxTalks is Changing
During Michaelmas Term, OxTalks will be moving to a new platform (full details are available on the Staff Gateway).
For now, continue using the current page and event submission process (freeze period dates to be advised).
If you have any questions, please contact halo@digital.ox.ac.uk
Forward genetic screens in haploid mammalian stem cells: looking beyond CRISPR/Cas9
Recent adaptation of the CRISPR/Cas9 bacterial system to facilitate manipulation of mammalian genomes has provided a real breakthrough for genome editing applications. Development of whole-genome CRISPR libraries with the aim of generating gene knockouts for every single coding sequence has allowed forward genetic screening in mammalian cells with unprecedented efficiency and versatility. CRISPR/Cas9 approaches, however, rely on phenotypes associated with loss-of-function mutations. Single-nucleotide variations (SNVs), on the other hand, have the potential to uncover not only loss-of-function phenotypes (by generating nonsense mutations, for example) but also gain-of-function phenotypes through missense mutations. In addition, they produce valuable information regarding functionally important domains of the affected gene product, as SNVs causative of a particular phenotype tend to cluster around specific regions of the amino acid sequence of the encoded protein. SNV-based approaches in mammalian cells, however, have been hindered by the diploid nature of their genomes, a fact that complicates the establishment of straightforward genotype-to-phenotype correlations. In this talk I will discuss how we apply CRISPR/Cas9 genetic screening to further understand the DNA-damage response in mouse embryonic stem cells (mESCs), and I will also introduce the use of haploid mESCs to perform SNV-based forward genetic screens.
Date:
16 July 2015, 14:30
Venue:
MRC Weatherall Institute of Molecular Medicine, Headington OX3 9DS
Venue Details:
Seminar Room
Speaker:
Dr Josep Forment (University of Cambridge)
Organising department:
MRC Weatherall Institute of Molecular Medicine
Organiser:
Penny Berry (University of Oxford, Department of Oncology, Weatherall Institute of Molecular Medicine)
Organiser contact email address:
penny.berry@oncology.ox.ac.uk
Host:
Dr Peter McHugh (University of Oxford, Weatherall Institute of Molecular Medicine)
Part of:
WIMM Occasional Seminars
Booking required?:
Not required
Audience:
Members of the University only
Editor:
Penny Berry
