Dendritic cells control the formation, maintenance, and function of tertiary lymphoid structures in cancer
In-person only
Tertiary lymphoid structures (TLS) correlate with favorable responses to immunotherapy, yet the mechanisms governing their formation and maintenance in tumors remain poorly defined. Through high-resolution spatial mapping across human tumors, we identified a consistent accumulation of CCR7⁺ mature dendritic cells (DCs) within TLS. To dissect their function, we developed a novel non-small cell lung cancer (NSCLC) model that recapitulates mature TLS formation. Using this model, we demonstrate that TLS induction requires IFNγ-driven maturation of type 1 conventional dendritic cells (cDC1), their migration to tumor-draining lymph nodes (TdLNs), and the subsequent recruitment of TdLN-primed T cells into the tumor microenvironment. As tumors advance, TLS persist even in the absence of TdLN T cell egress, coinciding with impaired cDC1 migration to TdLNs and their retention within intratumoral stromal hubs enriched in CCR7 ligands. Timed depletion of cDC1 or blockade of their localization to these hubs disrupts TLS maintenance. Strikingly, genetic ablation of both MHC class I and II on cDC1 abolishes the maintenance of TLS, T follicular helper (TFH) cells, germinal center formation, tumor-specific IgG production, and the differentiation of progenitor exhausted CD8⁺ T cells. These findings establish that local cDC1-mediated activation of CD8⁺ and CD4⁺ T cells are central orchestrators of anti-tumor TLS programs and compelling targets for therapeutic intervention.
Date: 17 April 2025, 10:00
Venue: Kennedy Institute of Rheumatology, Headington OX3 7FY
Venue Details: Kennedy Lecture Theatre
Speaker: Raphael Mattiuz (Icahn School of Medicine at Mount Sinai)
Organising department: Kennedy Institute of Rheumatology
Organiser: Magdalena Gross
Host: Mike Dustin
Part of: Kennedy Institute Seminars
Booking required?: Not required
Audience: Members of the University only
Editor: Magdalena Gross