Oxford Nanopore User Meeting

This meeting is for Oxford-based academic researchers who are interested in or involved in using Oxford Nanopore Technology. It has been set up by researchers at the Wellcome Centre for Human Genetics, in collaboration with Oxford Nanopore Technolgies, with the aims of:

- Getting a sense of the range of applications that ONT users within the university are involved in – Sharing experiences and ideas – Fostering fruitful connections and collaborations



9:00 – 9:10 Welcome from Dr David Buck, Head of the Oxford Genomics Centre, University of Oxford
9:10 – 9:35 “Through the Nanopore… An Introduction to Nanopore Sequencing” Ollie Hartwell, Strategic Account Manager UK & Nordics, Oxford Nanopore technologies.
Clinical sequencing on the PromethION platform

9:35 – 10:00 “Detecting clinically relevant structural variants with ONT PromethION Whole Genome Sequencing” Dr. Hannah Roberts & Maria Lopopolo, Oxford Genomics Centre, University of Oxford
10:00 – 12:00 “Nanopore-based genomic and transcriptomic sequencing of highly rearranged leukaemia genomes” Dr. Kiran Garimella, Big Data Institute, University of Oxford
Transcriptomics and base modifications

10:20 – 10:40 “Optimisation and evaluation of cDNA sequencing – the long and short of it” Dr. Eshita Sharma, Oxford Genomics Centre, University of Oxford
10:40 – 11:10 Coffee Break
11:10 – 11:30 “Investigating promiscuous splicing in thymic epithelial cells with ONT sequencing” Kathrin Jansen, Kennedy Institute of Rheumatology, NDORMS, University of Oxford

11:30 – 11:50 “The detection of 7-deazaguanine in RNA using nanopore sequencing: progress towards detection of naturally occurring G-quadruplexes” Dr. Carika Weldon, Oxford Genomics Centre, University of Oxford

11:50 – 12:50 Lunch, including demos and a sample clinic with Oxford Nanopore Michelle Hiscutt, Product Manager: Sample Preparation

Microbial sequencing: de novo assembly and genome replication dynamics

12:50 – 13:10 “Development of Nanopore-Based Methods for Whole Genome Sequencing of Hepatitis B Virus” Dr. Anna McNaughton, Nuffield Department of Medicine, Peter Medawar Building, University of Oxford

13:10 – 13:30 “Genome replication dynamics on individual ultra-long nanopore sequence reads”. Dr. Michael Boemo, Sir William Dunn School of Pathology, University of Oxford & St. Cross College

13:30 – 14:30 Guest speaker: “Long reads for large genomes”. Prof. Matt Loose, University of Nottingham

14:00 – 14:30 Panel discussion / Q&A

14:30 – 14:40 Closing remarks, Dr David Buck