OxTalks will soon move to the new Halo platform and will become 'Oxford Events.' There will be a need for an OxTalks freeze. This was previously planned for Friday 14th November – a new date will be shared as soon as it is available (full details will be available on the Staff Gateway).
In the meantime, the OxTalks site will remain active and events will continue to be published.
If staff have any questions about the Oxford Events launch, please contact halo@digital.ox.ac.uk
Quantitative mapping of genetic interactions (GIs), by simultaneously perturbing gene pairs then measuring the resultant phenotypes, is a powerful tool for understanding complex biological phenomena permitting the unbiased characterization of gene function and the mapping of functional modules and pathways. This discussion will present a GI analysis pipeline to study the functions of HIV host factors through pairwise knockdown of 360 host genes (representing 129,600 combinations) followed by infection with HIV. This work combines a luminescence-based viral infectivity assay to quantify HIV infection with a microscopy-based cellular analysis, allowing us to assemble GI maps of the host factor complexes and pathways mediating the early HIV lifecycle.
