"Mechanistic and structural insights into protein complexes that regulate DNA repair and mRNA stability"
Almost every eukaryotic mRNA has a poly(A) tail which is added by a 1 MDa multi-protein complex called Cleavage and Polyadenylation Factor (CPF/CPSF). There are four different enzymes in CPF (nuclease, polymerase and two protein phosphatases) but it is not understood how these are co-ordinated to define mRNA 3′-ends and regulate transcription. In addition, it is not clear what makes the poly(A) RNA sequence unique. We use a combination of structural biology (cryo-EM, X-ray crystallography, NMR) and biochemical reconstitution to address these questions and to gain mechanistic insight.
Date: 5 July 2019, 11:00 (Friday, 10th week, Trinity 2019)
Venue: Wellcome Trust Centre for Human Genetics, Headington OX3 7BN
Venue Details: Meeting rooms A & B
Speaker: Dr Lori Passmore (MRC Laboratory of Molecular Biology in Cambridge)
Organiser: Agata Krupa (Wellcome Centre for Human Genetics, University of Oxford)
Hosts: Prof Yvonne Jones (University of Oxford), Prof David Stuart (University of Oxford)
Part of: Strubi seminars
Booking required?: Not required
Audience: Members of the University only
Editor: Agata Krupa