"Mechanistic and structural insights into protein complexes that regulate DNA repair and mRNA stability"
Almost every eukaryotic mRNA has a poly(A) tail which is added by a 1 MDa multi-protein complex called Cleavage and Polyadenylation Factor (CPF/CPSF). There are four different enzymes in CPF (nuclease, polymerase and two protein phosphatases) but it is not understood how these are co-ordinated to define mRNA 3′-ends and regulate transcription. In addition, it is not clear what makes the poly(A) RNA sequence unique. We use a combination of structural biology (cryo-EM, X-ray crystallography, NMR) and biochemical reconstitution to address these questions and to gain mechanistic insight.
5 July 2019, 11:00 (Friday, 10th week, Trinity 2019)
Wellcome Trust Centre for Human Genetics, Headington OX3 7BN
Meeting rooms A & B
Dr Lori Passmore (MRC Laboratory of Molecular Biology in Cambridge)
Agata Krupa (Wellcome Centre for Human Genetics, University of Oxford)
Prof Yvonne Jones (University of Oxford),
Prof David Stuart (University of Oxford)
Members of the University only