OxTalks will soon move to the new Halo platform and will become 'Oxford Events.' There will be a need for an OxTalks freeze. This was previously planned for Friday 14th November – a new date will be shared as soon as it is available (full details will be available on the Staff Gateway).
In the meantime, the OxTalks site will remain active and events will continue to be published.
If staff have any questions about the Oxford Events launch, please contact halo@digital.ox.ac.uk
Single-cell RNA-sequencing (scRNA-seq) methods have revolutionized the way scientists study the cell transcriptome. In this field, full-length scRNA-seq protocols stand as the gold- standard for sensitivity. After testing >400 reaction conditions, we developed FLASH-seq, the fastest and most sensitive scRNA-seq protocol currently protocol available. In our most recent work, we push the boundaries of this protocol to study and quantify isoforms, leveraging the recent advances in long-read sequencing technologies. The result is FLASH-seq-ONT, a method that combines the high-sensitivity of plate-based methods with molecule counting and true isoform characterization on the Oxford Nanopore Technologies sequencing platform.