Biomedical research often seeks to understand function through the use of computational models, and one of the linkages enabling this to happen is provided by microstructural imaging. In this talk, I will describe our progressive developments in extending imaging capabilities through the design and construction of novel imaging systems that seek to provide data at the scale and resolution required for integrated understanding. Our initial, and central, focus has been on structures that influence the electrical and mechanical function of the heart, but this has grown to encompass other tissues as diverse as lymph nodes, lungs and carotid bodies.