On 28th November OxTalks will move to the new Halo platform and will become 'Oxford Events' (full details are available on the Staff Gateway).
There will be an OxTalks freeze beginning on Friday 14th November. This means you will need to publish any of your known events to OxTalks by then as there will be no facility to publish or edit events in that fortnight. During the freeze, all events will be migrated to the new Oxford Events site. It will still be possible to view events on OxTalks during this time.
If you have any questions, please contact halo@digital.ox.ac.uk
The Nielsen group develops new methodologies enabling proteome-wide characterizing of underexplored post-translational modifications. Recently the group established a methodology that allows unbiased studies of endogenous ADP-ribosylation in a systems-wide manner. Here we describe usage of the Af1521 methodology for characterization of the ADP-ribosylation regulome as a function of cellular regulation based upon stimulus, time, and enzymatic perturbations. To this end, we identified and quantified thousands of ADP-ribosylation sites and determined their temporal dynamics after stimulating human cells with various types of genotoxic stresses, and profiled the regulation of the ADP-ribosylome upon cellular deletion of HPF1 and ARH3. We furthermore demonstrate the applicability of Activated Ion Electron Transfer Dissociation (AI-ETD) for improved detection and quantification of ADP-ribosylation, hereby advancing the study of ADP-ribosylation under physiological conditions and opening up new analytical directions for the integrative analysis of the ADP-ribosylation regulome. Collectively, we present a proteomics-based characterization of the ADP-ribosylation regulome and provide new insights into the global, integrative view of cellular regulation of this important protein modification.
