OxTalks will soon move to the new Halo platform and will become 'Oxford Events.' There will be a need for an OxTalks freeze. This was previously planned for Friday 14th November – a new date will be shared as soon as it is available (full details will be available on the Staff Gateway).
In the meantime, the OxTalks site will remain active and events will continue to be published.
If staff have any questions about the Oxford Events launch, please contact halo@digital.ox.ac.uk
The Nielsen group develops new methodologies enabling proteome-wide characterizing of underexplored post-translational modifications. Recently the group established a methodology that allows unbiased studies of endogenous ADP-ribosylation in a systems-wide manner. Here we describe usage of the Af1521 methodology for characterization of the ADP-ribosylation regulome as a function of cellular regulation based upon stimulus, time, and enzymatic perturbations. To this end, we identified and quantified thousands of ADP-ribosylation sites and determined their temporal dynamics after stimulating human cells with various types of genotoxic stresses, and profiled the regulation of the ADP-ribosylome upon cellular deletion of HPF1 and ARH3. We furthermore demonstrate the applicability of Activated Ion Electron Transfer Dissociation (AI-ETD) for improved detection and quantification of ADP-ribosylation, hereby advancing the study of ADP-ribosylation under physiological conditions and opening up new analytical directions for the integrative analysis of the ADP-ribosylation regulome. Collectively, we present a proteomics-based characterization of the ADP-ribosylation regulome and provide new insights into the global, integrative view of cellular regulation of this important protein modification.
