Oxford University Cortex club presents Dr Peyman Golshani: Seminar on New imaging tools to decipher neural network dysfunction underlying cognitive deficits in epilepsy.

Temporal lobe epilepsy is associated with memory deficits but the circuit mechanisms underlying these
cognitive disabilities are not understood. We used electrophysiological recordings, open-source wire-free
miniaturized microscopy and computational modeling to probe these deficits in a model of temporal lobe epilepsy.
We find desynchronization of dentate gyrus interneurons with CA1 interneurons during theta oscillations and a
loss of precision and stability of place fields. We also find that emergence of place cell dysfunction is delayed,
providing a potential temporal window for treatments. Computation modeling shows that desynchronization rather than
interneuron cell loss can drive place cell dysfunction. Future studies will uncover cell types driving these changes
and transcriptional changes that may be driving dysfunction. I will also discuss new miniaturized microscopy tools,
including wire-free, large-field-of view and miniaturized microscopes integrated with high channel count
electrophysiology recordings.