John Scott has been instrumental in defining of how second messenger signaling event are compartmentalized within the cell. Through his discovery of the A-Kinase Anchoring protein family, he has shown how protein kinases, phosphatases and phosphodiesterases are constrained within second messenger nanodomains. He will discuss the discovery and investigation of new disease causing mutations in PKA and AKAPs that underlie Adrenal Cushing’s syndrome and adolescent liver cancers.