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Imaging a single protein has been a long-standing aspiration for
advancing structural biology and with this various fields in natural
science. Here I will show that, sub-nanometer resolved images of
individual folded proteins have been obtained for the first time.
Electrospray ion beam deposition for the specific selection and sound
deposition of individual proteins onto ultraclean freestanding
graphene in an ultra-high vacuum environment and low-energy
electron holography for the non-destructive imaging are combined in
a novel experimental workflow.
