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Understanding information flows and their changes in the brain requires a comprehensive map of neural structures at all levels, similar to those of Google Earth for continents, countries, cities, and streets. By integrating multiscale imaging technologies, I propose a practical approach aiming for mapping individual neurons, cellular organelles, synapses and single molecules in the entire Drosophila brain. I will discuss how the generated connectome map helps us to classify cell types, predict information flows, and manipulate target neurons that orchestrate complex behaviors. Our long-term goal is to construct the Drosophila engram and understand how learning and memory change the decision.
