Beauty and Benefits of cryo-EM; our new endeavors in vitrification of proteins and cells

We develop new methods for sample application and vitrification and implement them into a novel platform. The apparatus can be fed with pre-mounted autogrids, circumventing the need for postmounting. For single particle analysis (SPA), minute amount of samples can be applied directly onto a supported film. For cellular work, sample carriers with cells grown on them will be exposed to an airknife that removes excess medium. Sample evaporation will be minimized by a feedback loop that maintains the sample near its dewpoint calculated from the ambient temperature and humidity. Vitrification will be accomplished by jet-streaming liquid ethane onto the centre of the grid. I will be happy to share our latest results.