Molecular Organisation of Cell Junctions in Epithelial Tissue

In person only

Understanding how does functional order emerges from individual components is a major challenge of current biology. It’s particularly fascinating how epithelial cells organize its components in space and time to shape adhesion mesoscale structures such that they keep a robust and functional architecture that enables tissue homeostasis and organ function. We initially revealed that the initiation of tight junctions is triggered by biomolecular condensates of the protein ZO-1 at the cell adhesion sites. Later, we discovered that a biophysical wetting phenomenon orchestrates the assembly of the tight junction around the apical interface sealing the tissue. We now aim to investigage the molecular assembly of cell-cell adhesions in epithelia tissue and in the pathology of intestinal diseases such IBD exploring biophysical mechanisms involving membrane-condensates. Combining cell biology, super-resolution STED microscopy and chemical biology, we aim to uncover how cells control different physico-chemical environments to drive self-organisation processes that shape mesoscale structures enabling tissue function.