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Holger will discuss strategies for quantitative bottom-up proteomics analysis of complex biological samples. Different approaches to data acquisition, including Data-Dependent Acquisition (DDA), Data-Independent Acquisition (DIA) and targeted proteomics analysis will be highlighted. With a focus on the DDA-based analysis technique, label-free and isotopic labelling approaches will be discussed. A particular focus will be placed on experimental design, handling of large data sets, data visualisation and statistical data analysis.
